Infrared fluorescence trichromes of plants (with diffuser)

[Fandyus]

Technique explained here:

https://www.ultravioletphotography.com/content/index.php?/topic/4872-ir-fluorescence-trichrome-photography/

 

The upgrade is that I used a diffuser this time, it fluoresces a lot when hit with the 395nm spotlight but I believe quite a bit of UV still gets through and hopefully the blue light generated does not affect the results too much. Otherwise it makes exposures much longer but at least the illumination is consistent.

While I have no idea what the practical use would be, I think the different colors are pretty fascinating, must be due to different concentrations of different chemicals in parts of the plant.

 

Crassula Marnieriana Hottentot:

 

 

 

 

 

 

 

 

 

 

[Andy Perrin]

Gorgeous results!

[colinbm]

Beautiful pascals.

[ulf]

Very nice!

I especially like the last image.

Good work!

[Foxfire]

By looking at the colored ones, had a thought that additionally to IR fluorescence, there might be also some coloring that is not related to it, but is happening similarly as if using different ND filters and stacking the BW images as RGB layers.

 

But the images are nice to look and compare. Have noticed that UV induced IR fluoresecence works nice with plants and fruits.

[Unscenerie]

makes me want to try this

so nice

[Andrea B.]

...had a thought that additionally to IR fluorescence, there might be also some coloring that is not related to it, but is happening similarly as if using different ND filters and stacking the BW images as RGB layers.

 

Yeah...could be. 

And also, I was wondering if the angle of the 3 different lights played a role. Here's a test:  Take one (only) of the lights and make 3 photos - 1st is illuminated from the right, 2nd is illuminated from the left, 3rd is illuminated from the top. Then trichrome them as usual. The fluorescence in each area should be/might be slightly different because different amounts of light are reaching the same areas on the plant/fruit. How does that show up in the trichrome? 

 

If there is some component based on the amount of light (your thought) or angle of light (my thought), then that does not negate the beauty of your technique. It simply makes for another variable to artistically control. 

 

To control any possible angle-of-lighting variable, I suppose you would have to set up a rigid placement of the LEDs. 

 

 

[Andy Perrin]

I really love the lighting, Fandyus! 

[Fandyus]

On 2/25/2024 at 10:46 PM, Andy Perrin said:

Gorgeous results!

Thanks!

On 2/26/2024 at 1:29 AM, colinbm said:

Beautiful pascals.

Thanks! Although I am not sure what you meant by pascals.

On 2/26/2024 at 9:57 AM, ulf said:

Very nice!

I especially like the last image.

Good work!

Thank you!

[Fandyus]

On 2/27/2024 at 10:15 PM, Foxfire said:

By looking at the colored ones, had a thought that additionally to IR fluorescence, there might be also some coloring that is not related to it, but is happening similarly as if using different ND filters and stacking the BW images as RGB layers.

 

But the images are nice to look and compare. Have noticed that UV induced IR fluoresecence works nice with plants and fruits.

Yes, UV makes plants glow red and infrared pretty well. Oddly enough even green will do that to a much lesser extent (as seen in the images).

As for contamination, there might be some. I am not sure how it could work but the images for the green fluorescence channel tend to be a lot more washed out. I think either some of the light is leaking through the cooler of the spotlights and is not being filtered by the TSN575 I use, also the diffuser might be fluorescing on its own, it most likely is. I can do the images without it but if I do that, the lighting is very uneven. Also keep in mind that the green light diffuses into objects (and namely green plants) much easier, so it causes an odd subsurface scattering effect.

[Fandyus]

On 2/29/2024 at 4:14 PM, Unscenerie said:

makes me want to try this

so nice

Thanks! You can get to it easily, the spotlights are sold for cheap on the likes of AliExpress, all you need then is some putty, filter rings to attach to the spotlights and mount IR blockers on, and preferably a diffuser to diffuse the spotlights because they will all be slightly different.

[Fandyus]

On 3/1/2024 at 2:34 AM, Andrea B. said:

...had a thought that additionally to IR fluorescence, there might be also some coloring that is not related to it, but is happening similarly as if using different ND filters and stacking the BW images as RGB layers.

 

Yeah...could be. 

And also, I was wondering if the angle of the 3 different lights played a role. Here's a test:  Take one (only) of the lights and make 3 photos - 1st is illuminated from the right, 2nd is illuminated from the left, 3rd is illuminated from the top. Then trichrome them as usual. The fluorescence in each area should be/might be slightly different because different amounts of light are reaching the same areas on the plant/fruit. How does that show up in the trichrome? 

 

If there is some component based on the amount of light (your thought) or angle of light (my thought), then that does not negate the beauty of your technique. It simply makes for another variable to artistically control. 

 

To control any possible angle-of-lighting variable, I suppose you would have to set up a rigid placement of the LEDs. 

 

 

My LEDs are placed rigidly, Andrea. I use an adjustable lamp with an e27 fitting, I take care to not move it between shots. There is a few mm it moves each time because I have to go and physically unscrew the spotlights but that's about it.

[colinbm]

Oh dear, I must have been asleep when I wrote this ?
I meant, beautiful pastel colours.