Andrea B. Posted February 19, 2023 Share Posted February 19, 2023 This is a nice poster from the www.compoundchem.com. The poster is being used under Createive-Commons license with no changes and with attribution. And of course we are a non-commercial website. No details here about what wavelength of UV induces the protoporphyrin IX fluorescence. And only the most basic explanation of fluorescence. But still cool with that fluorescing red egg. Link to comment
Kai Posted February 20, 2023 Share Posted February 20, 2023 I can use it for educational purposes. Thank you Andrea :) Link to comment
Shane Posted February 20, 2023 Share Posted February 20, 2023 Porphyrin is excited by a broad emission wavelength ranging from UV to blue light. Link to comment
Doug A Posted February 22, 2023 Share Posted February 22, 2023 Cool poster @Andrea B.. Helps explain what is going on. Wish I could take one of @Kai's classes, they sound so interesting. Thanks for sharing, Doug A Link to comment
enricosavazzi Posted February 22, 2023 Share Posted February 22, 2023 On 2/19/2023 at 2:30 AM, Andrea B. said: [...] No details here about what wavelength of UV induces the protoporphyrin IX fluorescence. [...] Perhaps this helps: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4295789/ According to Fig. 3, there is an absorbance peak around 405-410 nm and an emission peak at 635 nm. Most likely the absorbance peak is where the energy enters the system. The emission peak is obviously where it leaves the system (plus any additional emission, see also below), and the difference between the two is the Stokes wavelength shift. Any leftover energy after the Stokes shift may leave the system at higher wavelengths (i.e. additional emission peaks), or ultimately as heat. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254220/ Fig. 1 only shows the same emission peak at 635 nm. Fig. 4 shows a second emission peak at 700 nm, which we could try to record with a VIS-cut, NIR-pass filter with transmission shoulder around 650-680 nm. An interference filter with a sharp shoulder should work, but not our typical absorbance NIR filters with very gradual shoulders. Link to comment
Unscenerie Posted February 23, 2023 Share Posted February 23, 2023 This is very interesting. Link to comment
Andrea B. Posted February 24, 2023 Author Share Posted February 24, 2023 https://www.ultravioletphotography.com/content/index.php?/search/&q=eggs&quick=1 We have several Egg topics here on UVP under the attached linkie. Link to comment
Adrian Posted March 5, 2023 Share Posted March 5, 2023 Great poster. It is interesting how different species of birds egg fluoresce different colours. Here: from top, chicken, duck and quail. Link to comment
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