colinbm Posted February 20, 2023 Share Posted February 20, 2023 A Pollen 100 x magnification + 100% crop. Link to comment Share on other sites More sharing options...
ulf Posted February 20, 2023 Share Posted February 20, 2023 How did you define the width of that size ruler? That can be a bit complex if not calibrated with a finite conjugate microscope objectives like the 100x objective you use. The magnification depends on the distance between objective and camera sensor. Here is a cheap calibration slide that you might want to get and try: https://www.ebay.com/itm/273445014429? It is chrome pattern on the glass slide's surface and you must use it with the chrome pattern facing the objective. Maybe the slide should rest on a white surface to get the pattern visible at all Link to comment Share on other sites More sharing options...
colinbm Posted February 20, 2023 Author Share Posted February 20, 2023 Thanks Ulf, I am glad you like it. I have a calibration slide with 0.1 divisions. The full frame at 36mm was 0.35mm wide, with 6000 pixels. In IrfanView you can measure pixels & the pollen was 440 pixels wide, so 6000 / 440 = 14, therefore 0.35mm / 14 = 0.025mm. Link to comment Share on other sites More sharing options...
photoni Posted February 20, 2023 Share Posted February 20, 2023 @colinbm You were very good I googled the various types of pollen, but I found many ugly and blurry photos. I believe that "thorny" types like yours are from fir trees. (for me the "ordeal" of spring has begun, I am very allergic to parietaria, hazelnut, alder, and grasses; the pollen seems smaller and smoother) Link to comment Share on other sites More sharing options...
colinbm Posted February 20, 2023 Author Share Posted February 20, 2023 The plant is a lawn perennial & has tiny 3-4mm flower heads, not unlike the Dandilion, but much smaller, but grows up to 1.5m if left unchecked. Here is the top 600mm. One of the flower heads. Link to comment Share on other sites More sharing options...
nfoto Posted February 20, 2023 Share Posted February 20, 2023 A member of the Asteraceae, maybe Conyza canadensis or similar. Link to comment Share on other sites More sharing options...
colinbm Posted February 20, 2023 Author Share Posted February 20, 2023 4 minutes ago, nfoto said: A member of the Asteraceae, maybe Conyza canadensis or similar. Thank you Birna. Link to comment Share on other sites More sharing options...
colinbm Posted February 22, 2023 Author Share Posted February 22, 2023 @ulf Did I get the calculation correct ? Link to comment Share on other sites More sharing options...
ulf Posted February 22, 2023 Share Posted February 22, 2023 1 hour ago, colinbm said: @ulf Did I get the calculation correct ? Yes I think so. My fuzzy brain at 4 in the morning could mot find any error, when I first read your reply. This grain of pollen seamed quite small. I had an idea that they generally were bigger, but that was based on memories that are very old from my teenage days when first using a simple microscope. They can vary quite a lot in shape depending on how they are spread. Link to comment Share on other sites More sharing options...
Unscenerie Posted February 23, 2023 Share Posted February 23, 2023 I've never even hoped to see pollen in its full glory, thanks for that! Link to comment Share on other sites More sharing options...
colinbm Posted February 24, 2023 Author Share Posted February 24, 2023 @Unscenerie Thanks, I hope to do more & get improvements too. Link to comment Share on other sites More sharing options...
Andrea B. Posted February 24, 2023 Share Posted February 24, 2023 Yow, wow!! Thank you, Col. Love this pollen. ((No wonder I get an itchy nose when shooting flowers, ha-ha.)) Agree with Birna that this flower appears to be a Conyza aka "Horseweed". Link to comment Share on other sites More sharing options...
Andy Perrin Posted February 24, 2023 Share Posted February 24, 2023 That's very cool! Could you do one with fluorescence? They glow so brightly that I always wondered what they looked like up close in UVIVF! Link to comment Share on other sites More sharing options...
colinbm Posted February 25, 2023 Author Share Posted February 25, 2023 @Andrea B. @Andy PerrinThanks I am trying to deal with the flare ? I think the pollen is very bright & I have to under expose a lot to reduce the flare ? I am trying things out ? Yes I will do a UVIVF when I get sorted of not. Of interest though, I was trying things out before I went to bed & now this morning one of the pollen has changed colour from yellow to cyan ? Link to comment Share on other sites More sharing options...
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