dabateman Posted July 1, 2023 Share Posted July 1, 2023 Interesting. After my move I seemed to have messed up my 63x objective. My 40x still seems ok. Not sure what I did. My scope is also quite dirty now. One day I will have to see if I can clean it and get things working again. Link to comment
JMC Posted July 1, 2023 Author Share Posted July 1, 2023 29 minutes ago, colinbm said: Ok, it must be thin. At that magnification I am using 0.6um stacks & they seem too big ? Yes a very thin diatom, and even better this one was mounted flat as well. Usually I need to stack, but not here. Link to comment
JMC Posted August 1, 2023 Author Share Posted August 1, 2023 A stack from a slide of Synedra robusta. Done on my modified Olympus BHB microscope, using 365nm light (Nemo torch). 100x Leitz Pl Apo NA 1.32-0.60 objective, oil immersion. Reichert Neo 1.42/1.18 dark ground condenser, oil immersion. Objective iris closed down to make a dark ground image. 2.5x Nikon CF PL photoeyepiece. Monochrome converted Nikon d850 camera. 6 images stacked in Zerene. Reduced in resolution for sharing here (original image size 6808x4992). Also shown is a crop from the main image, again reduced in resolution for sharing. Link to comment
Andy Perrin Posted August 1, 2023 Share Posted August 1, 2023 Whoa, always exciting to see updates to this thread! That diatom looks like a machine part. Link to comment
Andrea B. Posted August 1, 2023 Share Posted August 1, 2023 It does indeed! So cool to see these. Thanks, Jonathan. BTW, I added the Macro tag. Link to comment
colinbm Posted August 2, 2023 Share Posted August 2, 2023 Fantastic Jonathan. Do these repeating nano structures have any iridescence ? Link to comment
JMC Posted August 2, 2023 Author Share Posted August 2, 2023 Thanks All. I've not done much with UV on the microscope recently, as often I just use 450nm (quite a lot of slide mountants block the UV even 365nm), but will share things as and when I can. Col, yes, you get some amazing effects with these repeating structures. With some diatoms as you move the stage up and down the holes go back and forth from light to dark (makes stacking a bit of a nightmare at times). Also I have some slides by Horace Dall which have had the samples coated in thin films of TiO2. They give some fanstastic colours as the film thicknesses of the TiO2 vary slightly across the sample. If anyone's interested, I've put some images from a famous slide maker called Klaus Kemp here - https://jmcscientificconsulting.com/microscopy-diatom-arrangements-by-klaus-kemp/. Klaus did amazing diatom arrangements. And there's some examples of Horace Dall's TiO2 coated slides here - https://jmcscientificconsulting.com/microscopy-horace-dall-titanium-dioxide-mounted-diatoms-new-images/ Link to comment
JMC Posted August 10, 2023 Author Share Posted August 10, 2023 An image of Amphipleura lindheimerii. Unknown slide maker. Olympus BHB microscope, 365nm light from a Zeiss HBO 50W mercury xenon lamp. 63x Leitz Pl Apo 1.4 objective, oil immersion. Olympus Abbe condenser closed down slightly, oil immersion (simple brightfield image). 2.5x Nikon CF PL photoeyepiece. Nikon d850 monochrome converted camera. Stack of 3 images in Zerene. Full image and a crop from the main image. Link to comment
colinbm Posted August 10, 2023 Share Posted August 10, 2023 Looking good Jonathan. What diffuser is used here please ? Link to comment
JMC Posted August 10, 2023 Author Share Posted August 10, 2023 Cheers Col. Good question. I'm not sure and let me explain. I've fitted a fused silica diffuser from Thorlabs (https://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=6337) but off hand I can't remember which one is in there. The background to the image has been cleaned up manually by me as well. Link to comment
colinbm Posted August 10, 2023 Share Posted August 10, 2023 Thanks Jonathan I hadn't thought of ground silica. I have been using thin film PTFE or Whitish silicone film. Link to comment
JMC Posted September 24, 2023 Author Share Posted September 24, 2023 Not UV this time, but imaged using 450nm LED light. Auliscus sto[e]ckhardtii from a slide by WA Firth. This one was fun to image, and stacked really easily. Modified Olympus BHB microscope. 450nm LED light. 63x Leitz Pl Apo NA 1.40 objective with oil immersion. Olympus Aplanat Achromat condenser, oil immersion, oblique. 2.5x Nikon CF PL photoeyepiece. Monochrome converted Nikon d850 camera. 22 images stacked in Zerene (Pmax setting). Shared at original pixel resolution. Link to comment
colinbm Posted September 24, 2023 Share Posted September 24, 2023 An excellent photo Jonathan, so sharp & clear. Is this cell about to divide ? Link to comment
JMC Posted September 24, 2023 Author Share Posted September 24, 2023 Thanks Colin. No this is just the structure of that particular diatom. I see what you mean though. They also make me think of magnetic fields with iron filings. Link to comment
dabateman Posted September 24, 2023 Share Posted September 24, 2023 Yes reminds me of cell division or a coin. Great image Jonathan. Link to comment
photoni Posted September 24, 2023 Share Posted September 24, 2023 @JMC great photo, I tried to reverse and contrast it to put on the desktop... WOW . Link to comment
JMC Posted September 24, 2023 Author Share Posted September 24, 2023 8 minutes ago, photoni said: @JMC great photo, I tried to reverse and contrast it to put on the desktop... WOW Thanks Photoni. 1 hour ago, dabateman said: Yes reminds me of cell division or a coin. Great image Jonathan. Cheers David. Link to comment
JMC Posted November 22, 2023 Author Share Posted November 22, 2023 Some more images taken using 365nm light on my Olympus BHB microscope. Images have been reduced to 1600 pixels along the long side for sharing. Cymatopleura elliptica from Toomebridge, Ireland. A Gomphomena, again from Toomebridge. Arachnidiscus deficiens from Jackson's Paddock, Oamaru, New Zealand. A fragment of Biddulphia pedalis from Oamaru, New Zealand. Link to comment
Andy Perrin Posted November 22, 2023 Share Posted November 22, 2023 Nice pics! You seem to end up in UV-A a lot now. Link to comment
JMC Posted November 22, 2023 Author Share Posted November 22, 2023 13 minutes ago, Andy Perrin said: Nice pics! You seem to end up in UV-A a lot now. Thanks Andy. Yes 365nm allows me to use standard optics and standard slides, and the camera is still quite sensitive, so low noise and less vibration. As such it offers benefits for resolution above and beyond visible light, without having the drawbacks of going to shorter wavelengths. Don't get me wrong, I still want to get a good image using 254nm light and a high NA objective, but that is more of an academic exercise really - to see if I can do it. Link to comment
colinbm Posted November 23, 2023 Share Posted November 23, 2023 Nice work Jonathan Are these single or stacked images ? Link to comment
JMC Posted November 23, 2023 Author Share Posted November 23, 2023 9 hours ago, colinbm said: Nice work Jonathan Are these single or stacked images ? These were all stacked in Zerene. Link to comment
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