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Same settings and equipment as the other flowers in this series, with exceptions noted below. UV 2mm UG11 + 1.75mm S8612 (Convoy S2+) Saturation strongly increased Visible BG38 2mm and DB850 filter (645-405nm) NIR-Red-Green (550-645nm + 835-875nm) Tiffen#12 + DB850 filter, and my usual algorithm for making the IRG, described in the DB850 filter thread NIR Hoya R72 + DB850 filter (835-875nm) SWIR (1500-1600nm, made by the panorama method and reduced for higher resolution) UVIVF under Convoy S2+ and using BG38 2mm and DB850 filters --- Comments: The SWIR looks a lot like the visible but with a lighter disk in this one, enough that I got scared and decided to check if my filter was leaking. However when I stacked it with my hard-coated premium Thorlabs 1200nm long pass (guaranteed >OD5), it looked the same, so I guess it just looks similar by coincidence. If you look at the bottom left of the flower, there is a tiny splash of water on a petal, so you can see that the water, although dark, isn't inky when it's just a thin layer like that. This morning the disk florets had started to open up, and they are white in the SWIR 1500-1600nm band:

Botany Bay Weevil on a cornflower. Shot in Ultraviolet induced visible fluorescence. Canon 5D Mark IV , Canon 180mm F3.5L. 1/160 F16 ISO 8000

The Nemo flashlights arrived. Their special filters look similar to a ZWB1. What camera filters are they comparable to? When using the torches with supplied filter, is an UV filter still required on the camera lens? If so, can the UV filter be added to the flashlight? I walked around outside in the dark with a torch and found some nice subjects. So glad there aren't neighbors to explain the goggles and weird light to. Thanks, Doug A

My friend David Pelling recently showed me a pebble he had found at Charmouth beach, near Lyme Regis in Dorset, UK, with a small pair of fossil ammonites (possibly either Promicroceras or Dactylioceras sp.). These fluoresced well with 365nm UV light. In particular the small one shows far more detail than in visible light, showing up the chambers (septa) very clearly. Technical details: Nikon D850 with 105mm micro Nikkor lens. UVF: 10 seconds at f/22, light painted with NEMO torch.

Target UV & UV Grey Card, UVA Fluorescent Standards, Colour Checker. http://www.imagescienceassociates.com/mm5/merchant.mvc?Screen=PROD&Store_Code=ISA001&Product_Code=TUVUVGC&Category_Code=TARGETS I would like to have something like this, but the cost / benefit is unfavourable. I would love to know the minerals used ?

Oldfield, D. 2021. Diuris sulphurea R. Br. (Orchidaceae) Tiger Orchid. Flowers photographed in visible and ultraviolet light. https://www.ultravioletphotography.com/content/index.php?/topic/5066-diuris-sulphurea-tiger-orchid-another-specimen Maldon, Victoria, Australia 12 October 2021 Australian Native Wildflower as Garden Specimen Comment This specimen was grown from a tuber obtained from Nesbitt’s Orchids of Walkerville, South Australia, in February 2016. This was the first year that flowers were seen. Visible Light: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens, Metz 15 MS-1 flash, 1/200 s @ f/16 ISO 200, Baader UV/IR Cut Filter. Image Reference: DO67408 Ultraviolet Light: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens, Nissin Di866 Mark II flash, 1/200 s @ f/16 ISO 200, Baader UV-Pass Filter. Image Reference: DO67410 Ultraviolet Induced Visible Fluorescence: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens with Baader UV/IR Cut Filter, Nichia NCSU033A UV-LED with Baader UV-Pass Filter, 10.0 s @ f/16 ISO 1600. Image Reference: DO67411 Reference: Jones, D. L. A Complete Guide to Native Orchids of Australia Third Edition, Reed New Holland, 2021, p. 259. Published 2 December 2021

Hi all, I'm busy building a light source to use to create UVIVF time lapses similar to this example: UVIVF Time lapse - Youtube My main requirement is to achieve a high quality 365nm light for visual fluorescence photography, with enough power to give me flexiblity with exposure settings. I intend to shoot mostly flower time lapses in an enclosed light proof environment. My first build is 5x3Watt led chips from AliExpress with a similarly generic ZWB2 filter which I am still awaiting delivery on. Effieciency is not a major concern for me as long as heat doesn't become a problem. I'm using an actively cooled heatsink and the UV light will only be on for about 20seconds followed by at least 40seconds off. As good quality components (Nichia LED's, Hoya/Schott filters) are at least 10x the price of what I used and I'm trying to keep costs maneagable, my question is where should I invest more if required? Ie would I be better off using the cheap LED's and then trusting a high quality filter to block unwanted wavelengths or rather use Nichia chips with a generic Filter? Or should what I'm doing with work fine? Once I've finished my build I will post some photos of the results.

Hi All, I thought lets try some lovely UVIVF. Those pictures are my first one. I love this. Nikon D800, Nikkor 24-85mm f/2.8-4D IF, With unknown brand UV-filter, VIS Nikon D800, Nikkor 24-85mm f/2.8-4D IF, With unknown brand UV-filter Home made torch LEDs 365nm, ZWB2 Nikon D800, Nikkor 24-85mm f/2.8-4D IF, With unknown brand UV-filter Home made torch LEDs 365nm, ZWB1 And some lovely glasses from different types of glass. and some straws.. :-D Nikon D800, Nikkor 24-85mm f/2.8-4D IF, With unknown brand UV-filter Home made torch LEDs 365nm, ZWB2

Visible Uvivf I am going back when it gets dark to this garden to pinch a better bit :P

Oldfield, D. 2021. Grevillea longistyla W.J. Hooker (Proteacea) Long-style Grevillea. Flowers photographed in visible and ultraviolet light. LINK Maldon, Victoria, Australia 12 October 2020 Australian Native Wildflower as Garden Specimen Comment Grevillea longistyla occurs on sandstone ridges on the Blackdown Tableland of Queensland. Visible Light: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens, Metz 15 MS-1 flash, 1/200 s @ f/16 ISO 200, Baader UV/IR Cut Filter. Image Reference: DO66891 Ultraviolet Light: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens, Nissin Di866 Mark II flash, 1/200 s @ f/16 ISO 200, Baader UV-Pass Filter. Image Reference: DO66892 Ultraviolet Induced Visible Fluorescence: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens with Baader UV/IR Cut Filter, Nichia NCSU033A UV-LED with Baader UV-Pass Filter, 10.0 s @ f/16 ISO 1600. Image Reference: DO66894 Reference: Olde, P and Marriott, N. The Grevillea Book, Volume 2, 1995, Kangaroo Press, p. 245 Published 21 November 2021.

Oldfield, D. 2018. Conostylis candicans Endl. (Haemodoraceae) Grey Cottonheads. Flowers photographed in visible and ultraviolet light. http://www.ultraviol...ey-cottonheads/ Maldon, Victoria, Australia 21 October 2017 Australian Native Wildflower as Garden Specimen Synonym Conostylis albicans Benth. Comment Conostylis candicans has a wide distribution from Shark Bay north of Carnarvon to south of Perth on the coast of Western Australia. Visible Light: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens, Metz 15 MS-1 flash, 1/200 s @ f/16 ISO 200, Baader UV/IR Cut Filter. Image Reference: DO65005 Ultraviolet Light: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens, Nissin Di866 Mark II flash, 1/200s @ f/16 ISO 200, Baader UV-Pass Filter. Image Reference: DO65007 Ultraviolet Induced Visible Fluorescence: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens with Baader UV/IR Cut Filter, Nichia NCSU033A UV-LED with Baader UV-Pass Filter, 10.0 s @ f/16 ISO 1600. Image Reference: DO65008 References: Elliott, W.R. and Jones, D.L. Encyclopaedia of Australian Plants suitable for cultivation, Volume 3, 1984, Lothian, p. 75. Published 2 February 2018

Recently I tried to get some order into older images, which of course is a daunting task. However once in a while one discovers hidden treasures. One of these would be the flowers of Malva moschata Musk Mallow, Malvaceae. This is a species becoming increasingly naturalised on open grasslands and road verges in the lowlands. Flowers are pink or almost white. The stamens are connate and form a column in the flower centre through which the styles emerge. (Fuji S3Pro, UV-Nikkor 105/4.5, UV/IR blocking filter + BG-38) In UV the corolla is bright blue with black veins and the centre is dark as would be expected. (Nikon D3200/built-in Baader U, UV-Nikkor 105/4.5) The standard UVIVF capture shows the usual lint issue on the corolla and that something is going on in the centre. (Nikon Df, Laowa 100mm f/2.8 macro lens, NEMO torch) Time has come to increase magnification, to 5X. Still UVIVF. (Nikon Z7, Laowa 25mm f/2.8 at 5X, NEMO torch) Being ever so curious, I went all out to 20X magnification and got this, (Nikon Z7, Mitutoyo 20X/0.42 objective on an infinity-focus system, NEMO torch) The fact that some pollen grains fluoresce brightly, others don't, may indicate that the strong fluorescence is a result of pollen damage.

The following experiment inspires my students again and again. A real highlight ;) Thin branches of Fraxinus excelsior and Aesculus hippocastanum are cut lengthways and placed in water. Clouds of extracted aesculin (bluish-white) and fraxin (green-white) then fluoresce nicely, especially if you stir very slowly. According to Wikipedia (German entry), these compounds (gucosides of coumarin derivatives) were the starting point for the development of modern optical brighteners.

Lighting 2x Yongnuo yn560iii with Hoya U-340 and Schott S8612. Camera Canon 5D Mark IV , Tamron 90mm with Hoya HMC Super UV 0 filter.

First UVIVF picture post. I live in the woods and this was taken about 15 feet from my front door. Didn't have all the equipment worked out yet. It is very difficult to aim ballhead mounted camera and hold NEMO torch at the same time. I have since devised a hotshoe mount for the NEMO and added a Tiffen UV2 filter. Still adjusting to the yellow googles. Waved the NEMO around for 30 seconds at F11 ISO 100. Eventually I will try painting with two torches. Stock Pentax 645Z with Pentax 645 A 120 macro lens. Comments welcome. Thanks for looking, Doug A

Played with wb until I liked it.

Fluorescent Chicken Eggs, the Next Step. Some have posted pictures of Chicken Eggs in UVA Induced Visible Fluorescence. Here is a example of what I have seen taking things a bit further. The fluorescent component is protoporphyrin IX (and maybe some other porphyrins). You can extract it from the broken shells using vinegar, isopropyl alcohol and a little bit of salt.

Fluorescent light photo using the M42 Kuribayashi 50mm f2 A.C. from the original Petri Penta SLR. Captured using a 365nm torch and standard unmodified Sony a6300. The black eyed susan was sitting on the desk for a couple days with the stem in some wet towel. Over time the newly erupted pollen had sprinkled onto the leaf petals. There was also some strange weeping on the outer section of the flower petals just past where the usual central (UV absorbing) black white spot occurs. I don't know if this was a sign of rot or just leaf damage but you can also see it on the tip of a petal about 5 o'clock. This also shows up as an aqua color in some areas. ISO 100 1.5s f11 Enjoy, B1

The last 5 years or so I have been deeply committed to an Aquatic Plant Project financed by the Norwegian Ministry of Environment. Plain old-fashioned field botany skills are of course what is required in the sampling stages, and patience to extract oneself when sinking into subsurface bottoms or sticky mud is a advantage as well. The photography later "only" calls for stamina and willingness to develop high-magnification setups. Having a large porch where I can keep my not-always-nice-smelling samples helps too. Anyhow, all along I had ulterior motives as I saw the opportunity not only to collect the rarest and most elusive species in the country, but also could look into UV reflective photography (for the rather few species not having wind- or water-pollinated flowers), get familiar with aquatic ecosystems all over the country and their particularities, and last but not least, delve into stuff like finding parasitic fungi on my aquatics. Of the latter extremely little is know on the species present or their distribution. The water-plantain Alisma plantago-aquatica is a common species thriving in a wide range of aquatic systems from nutrient-poor to the very nutrient (eutrophic) ones. There is a curious dwarf form of it in a lake not very far away from my home, so I tend to make a visit there every second year or so. The systematic position of this form is unsolved and in fact it might even represent another species, but the jury is still out on that issue. This autumn I availed myself of a long dry spell conveniently making water levels drop in that lake, so access to the plants was facilitated. This is the dwarf Alisma on a laid dry lake floor. It occurs in a highly diversified plant community with many species at least one of them is endemic to Scandinavia. (Alisma are the broad-leaved rosettes) I had already found a smut fungus on Alisma in other locations. This smut, Doassansia alismatis, makes discoloured leaf spots in the late summer and autumn and within the leaf spots there are tiny spore balls embedded under the epidermis. The balls are 80-200 µm across, so just about discernable by the naked eye if you have 20/20 vision. Here is a typical spore ball, in fact a really big one as the scale indicates. The large cells are epidermis ("skin") of the leaf. Thus, when I found some leaf spots on the dwarf Alisma, I immediately considered this is a strange Doassansia. Strange, because the leaf spots were almost invisible and in fact, although I spent a long time dissecting the leaf under my binocular microscope, I didn't see the now so familiar spore balls at all. Curiouser and curiouser. I had but some small fragments of foliage left after the dissecting marathon., hence time to haul out the NEMO torches and have a look if there was something to be found and not only an optical illusion. Her is what I got with UVIFL approach. (scale with mm steps) The brightly fluorescing spots were 1-2mm. I then very carefully scraped off the surface within a few spots, stained the residue with Lactophenol Cotton Blue, and examined under the microscope. Again, no spore balls, but to my surprise there were masses of tiny, spindle-shaped conidia spores. So my enigmatic fungus was not a smut at all. Here is what I observed at 1000X (100x/1.25 Oil objective), (scale with 1µm steps) Consulting the literature I quickly found this was Plectosphaerella alismatis (Oudemans) Phillips, Carlucci & Raimondo, 2012. It belongs to the Ascomycetes: Fungi > Ascomycota > Pezizomycotina > Sordariomycetes > Glomerellales > Plectosphaerella > Plectosphaerella alismatis. Never found in my country before. And I learned two important lessons, firstly not to believe I knew the answer before having obtained sufficient details, and secondly, that UVIVFL can really assist in discovering biological phenomena. I had never found this species without UVIVFL assistance.

Oldfield, D. 2021. Acianthus pusillus D.L. Jones (Orchidaceae) Small Mosquito Orchid. Flowers photographed in visible and ultraviolet light. LINK Maldon, Victoria, Australia 2 May 2021 Australian Native Wildflower as Garden Specimen Comment Acianthus pusillus is endemic in Eastern Australia where it is widespread and common from coast to ranges growing in a wide range of forested and heathy habitats. Visible Light: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens, Metz 15 MS-1 flash, 1/200 s @ f/16 ISO 200, Baader UV/IR Cut Filter. Image Reference: DO67088 Ultraviolet Light: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens, Nissin Di866 Mark II flash, 1/200 s @ f/16 ISO 200, Baader UV-Pass Filter. Image Reference: DO67094 Ultraviolet Induced Visible Fluorescence: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens with Baader UV/IR Cut Filter, Nichia NCSU033A UV-LED with Baader UV-Pass Filter, 10.0 s @ f/16 ISO 1600. Image Reference: DO67095 Reference: Jones, D.L. A Complete Guide to Native Orchids of Australia, third edition, 2021, Reed New Holland, p. 46 Published 3 November 2021.

Some time ago me and my family picked mushrooms in the forest. I took some afterwards to experiment a little. UVIVF and last but not least, multispectral, something a little different. R-950nm longpass, B- ZWB2+QB39 with a fluorescent blacklight, G - visible

Technique explained in previous post. IR reference (720nm longpass) visible reference fluorescence under green fluorescence under blue fluorescence under 395nm trichrome (the "dust" is contamination on my PTFE sheet, which this shell was laid on)

So recently, I have developed a new (maybe) way to investigate the properties of objects. I'm not sure if it's been tried here before, but what I do is that I use three lightsources, each of different wavelength, l filter these lightsources with a QB39, and on the camera I mount a 720nm longpass and a 650nm longpass. The lightsources I use are generic chinese made spotlights that you find on eBay or Aliexpress. https://www.ebay.com/itm/164732255359?var=464226938551 https://www.aliexpress.com/item/4000068864312.html?spm=a2g0s.9042311.0.0.27424c4dpYRypM I use the blue, green and 395nm variety. The light causes the given object to give off fluorescence but its amount and wavelength differs slightly depending on the wavelength. I then stack the images and map them to different channels to illustrate the differences. The 650nm longpass is used in tandem in order to weed out the little green light that leaks through the 720nm longpass. I have prepared two examples, an apple, and a decaying leaf. leaf reference leaf under green leaf under blue leaf under 395nm leaf trichrome apple under green apple under blue apple under 395nm apple trichrome bonus: apple UVIVF If you have any suggestions of what else I should do, please do tell. I have tried a few other things, including pumpkin seeds, a mineral rock, the outside of an apple or the print on a juice box to see how the different dyes and bare paper behave. I might post that later. So far I love the results, I think they're really interesting and rich in how the color varies, which cannot be said for many other techniques here, such as UV, which really only gives around 3-4 colors depending on your reach. Next step for me is to make a setup with three different wavelength UV LEDs to make UV trichromes, I think that's a superior way to see into UV as it can give a full spectrum of colors and works in a way our brains understand. But enough rambling, hope you enjoy the post.

Oldfield, D. 2021. Orthrosanthus multiflorus Sweet (Iridaceae) Morning Flag. Flowers photographed in visible and ultraviolet light. https://www.ultravioletphotography.com/content/index.php?/topic/4873-orthrosanthus-multiflorus-morning-flag/ Maldon, Victoria, Australia 26 September 2021 Australian Native Wildflower as Garden Specimen Synonym Orthrosanthus multiflorus var. hebecarpa Benth. Comment Orthrosanthus multiflorus is a tufted perennial herb which occurs in south-western Victoria, on Kangaroo Island and the Eyre Peninsula in South Australia and from Israelite Bay to the Stirling Range in Western Australia. Visible Light: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens, Metz 15 MS-1 flash, 1/200 s @ f/16 ISO 200, Baader UV/IR Cut Filter. Image Reference: DO67336 Ultraviolet Light: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens, Nissin Di866 Mark II flash, 1/200 s @ f/16 ISO 200, Baader UV-Pass Filter. Image Reference: DO67338 Ultraviolet Induced Visible Fluorescence: Nikon D750 Full Spectrum Modification, Nikon Rayfact PF10545 MF-UV 105 mm f/4.5 lens with Baader UV/IR Cut Filter, Nichia NCSU033A UV-LED with Baader UV-Pass Filter, 10.0 s @ f/16 ISO 1600. Image Reference: DO67339 References: Elliott, W.R. and Jones, D.L. Encyclopaedia of Australian Plants suitable for cultivation, Volume 7, 1997, Lothian, p. 118. Walsh, N.G. and Entwistle, T.J. (eds) Flora of Victoria, Volume 2, 1994, Inkata Press, p. 692 Published 2 October 2021.